A Review on Eco-Friendly Formulation of Silver Nanoparticles and its Activity

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A Review on Eco-Friendly Formulation of Silver Nanoparticles and its Activity

Devi Bala.G1

1 Department of Biotechnology,

Vivekanandha College of Engineering for Women (Autonomous)

Dheshika. R2

2Department of Biotechnology,

Vivekanandha College of Engineering for Women (Autonomous)

Girija. M3

3 Department of Biotechnology,

Vivekanandha College of Engineering for Women (Autonomous)

Archana. A*

*Assistant Professor, Department of Biotechnology

Vivekanandha College of Engineering for Women (Autonomous)

Abstract:-Silver had been identified as a harmless, safe,in-organic compound and had been used as an anti-bacterial/anti-fungal agent over decades. Silver had extensive application in various domains including pharmaceutical and chemical industries especially in nanoparticle form due to its efficient activity. The size of the nanoparticles varies from 1-100nm. There is numerous method available for the formulation of nanoparticles but they are not convenient and economic and also the reactives used for formulation of silver nanoparticles can cause ecological impairment. presently the researchers had a thirst in this area by a formulation of nanoparticles from naturally available resources in a most effective manner and also is an economic way. This article precise the various green-mediated formulation of silver nanoparticles and their morphology characterization, anti-microbial activity.

Key Words: Harmless, Nanoparticle, green-mediated, anti-microbial, Economic, morphology characterization


The term nanotechnology is characterised as structure creation, characterization, and application by changing its size and shape,[1] commonly, the size of the nanoparticles ranges from 10-100nm in diameter. It is broadly classified into two types as natural and inorganic nanoparticles The nanoparticles silver, gold, titanium, zinc and copper are grouped into organic metals and the carbon nanotubes and quantum dots are grouped into in-organic metals. Among these various nanoparticles, silver nanoparticle(AgNPs) had a unique attraction due to its metallic and biochemical activity. AgNPs also used in various applications including pharma-industry, Biotechnological, and Food industry in versatile research approaches. During Centuries, before the evidence of penicillin activity, silver had been used as a therapeutic agent. As the silver comes in nanosize (less than

100) due to their size reduction its shows considerable activity against different microorganisms besides Gram-positive and Gram-negative bacteria. In recent years the silver nanoparticles also had a great focus in the agricultural field it was used as a fertilizer as well as pesticides.Due to their nano-size, the particles can easily pass through the cuticle of the plant and showed a

controlled realized activity.there are diverse methods available for the formulation of nanoparticles (physical, chemical, microbial). But they are not cost-effective and also the chemicals and microbes used for the formulation of silver nanoparticles cause harm to the ecosystem. In the development of the field of nanotechnology, the researcher had used various naturally available resources for the formulation of nanoparticles, for example, oil-cake, leaf extract, fruit extract acts as a reduction agent and capping agent. The nanoparticles synthesized by thesis methods show a prominent activity and also it becomes cost-effective compared to other chemical and microbial formulation. The researchers reported that the naturally available phytochemical compounds for example Alkaloids, flavonoid, terpenoids, saponin, etc.., present in the extract act as a reduction agent that reduces the metallic silver into a metal nanoparticle.Silver nanoparticles also used for biofilm formation and in wastewater treatment for heavy metals removal and also for the micro-organism trapping to purify the water for various utilizing activities. This review focuses on the various green-mediated formulation of AgNPs their biochemical activities and their characterization technique


Green formulation of AgNPs using Azadirachta indica

The leaf extract of Azadirachta indica,( in Indian language is called neem)is used for the environment-friendly formulation of nanoparticles,[2]. The researcher had[2,3] reported that the high amount of flavonoid and terpenoids compounds presence in leaf extract act as a reducing agent that reduces the Ag+ ion into AgNPs particles. The researcher mixed the leaf extract and 1Mm AgNO3 in different concentrations at test tubes by brown color formation the formulation of nanoparticle had identified and observed OD at 200-800nm [3]. The analyzer reported that the very high concentration of leaf extract is not suitable for nanoparticle formulation and identified that one-third proportion of sample and the AgNO3 showed prominent antibacterial activity against E.coli and other gram-positive bacteria with zone formation of 20l-8.66mm; 50l-18.66mm; 100l-22nm.

Green formulation of AgNPs using piper longum (long pepper)

AgNPs synthesized by using Piper longum displayed extreme cytotoxic activity against the hep-2 cell line [4]. 90ml of 1Mm aqueous solution of AgNO3 and 3 ml of leaf extract was added. The mixer kept in dark and maintained by room temperature. The formation of yellow colour suggested the emergence of silver nanoparticles and the absorbence was estimated at 200 to 600 nm. The Nanoparticles were produced at various sizes 17.6nm, 29.3nm,31.6nm,37.5nm depending upon their sample and AgNO3 mixing proportions. And,[3] identified that the notable cytotoxic effect was showed in 500g/ml concentration of AgNPs in that 51% of the hep2-cell line were died.

Green formulation of AgNPs using leaf extract of Spartium junceum

The Spartium junceum solution used for nanoparticle formulation[5]. And the researcher proceeded 25ml of 1mM AgNO3 was added into 25 ml of S.junceum solution. The mixture was heated for 24 hours at 800c. By the observation of color changes from yellow to brown indicated the existence of silver nanoparticles,[6]. The morphological characters were analyzed by SPR, UV-Spectrophotometer and FTIR [7].

Green formulation of AgNPs using Impatiens-balsamia and Lantana camara

The leaf extract of Impatiens balsamia and Lantana camara used as a reduction agent for the formulation of AgNPs [8]. Different concentrations of AgNO3 stock solution were prepared (1mM, 2mM, 3mM,) and the leaf extract of I.balsamina and L.canara was added to it and the solution was heated at 60oC for 5 hours. The nanoparticle formulation indicates the observation color change [9]. The researcher identified that the nanoparticles synthesized by these leaf extract showed high anti-microbial activity with the zone of inhibition from 11.03 mm to 13.08 mm.[10,11]

Green formulation of AgNPs using Amaranthus viridis

Amaranthus viridis, cosmopolitan species commonly called as slender amaranth (in Indian language), leaves and seeds were edible and are used as vegetables and tops they are high in calcium, iron and found to be bonne of vitamin B and Vitamin C sources, it also showed anti-diabetic and anti-hyperlipidaemic activity [12]. Amaranthus viridis had used for large scale production of nanoparticle formulation had been reported. Leaves were collected, washed thrice in distilled water then washed with Milli Q water to remove water, leaves were dried in room temperature for about 2h, then 5grms of leaves were chopped and boiled with Milli Q water for 50°C at 10 minutes, then extract using Whatman N.1 filter paper. 1mM of AgNO3 (0.0421g) dissolved in 250ml of Milli Q water in the orangey colored bottle. AgNPs were synthesized by dissolving 5ml of leaf extract in 20ml of 1mM of AgNO3 and heated at 100°C, the color turns light yellow to reddish-brown confirms AgNPs present in the extract. Synthesized AgNPs were characterized under FTIR shows band range of 3423-2850nm, Scanning Electron Microscopy

Energy,Dispersive Spectroscopy,(SEM-EDX)Transmission, and Electron Microscopy which measures the size of the band ranges from 5-20nm, XRD.[13] The stability of reduced AgNPs analyzed by UV-Visible spectroscopy, peak observed at 4235nm. As compared to other sources, Amaranthus viridis highly effective and useful, antibacterial activity against gram-positive and gram-negative, AgNPs from the Amaranthus viridis contains Oxides; Calcium; Silver.[14]

Green formulation of AgNPs from ceratonia siliqua (carob)

Simply known as 2 min reaction time at lukewarm for reduction of Ag+ ions to AgNPs. Leaves were washed with distilled water, dried, sliced into small pieces and boiled in distilled water for about 10 mins, filtered the extract which was in yellow then centrifuged to remove bulk bio-materials at 1200rpm for about 5 mins. [15] AgNPs were synthesized by dissolving 5ml of extract in 1mM of 100ml of AgNO3 the mixture changes into black light which confirms AgNPs. By comparing to other carob plants, Ceratonia siliqua shows a quickly synthesized one. The concentration of AgNO3 and leaf extract were changed from 1-4mM and 5%-10% by volume. The characteristics analysis of AgNPs involves UV-Visible spectroscopy shows strong resonance band of surface plasmon at 420nm [16] which indicated the formation of AgNPs, FTIR measures the band at 4000 to 400cm-1, SPR produced a peak near 420nm, XRD founds the crystalline size of AgNPs around 18nm which shows that the AgNPs are nanocrystalline, SEM measures the particle size ranges from 5-40nm. AgNPs could be controlled to 5-40nm by varying the extract volume and the AgNO3 leaf concentration were used as a reduction agent and highly effective against E.coli.[17]

Green formulation of AgNPs using Cynara scolymus


species of cyclamen, cultivated as a part of the food, leaf extract were known to show anti-oxidant, anti-inflammatory, anti-allergic, anti-ulcerogenic and anti-hepatocellular carcinoma activity and they are abundant in metabolites such as chlorogenic acid, luteoline, apigenin derivatives of cynarine acid and flavonoids. Leaves were collected washed thrice in deionized water, chopped into small pieces, 200gm of leaves were added in 400ml of deionized water were added to 1L of Erlenmeyer flask heated to 100C for 2h then filtered using Whatman filtered paper-1, 10mM of 20ml of AgNO3 in 100ml were added to 20ml of extract in a dropwise manner, color changes brown to orange, the shreds of evidence shows the formation of AgNPs.[18] The characteristic analysis of AgNPs includes UV-Vis spectroscopy measures the particle size in the range of 200-800nm, FTIR analysis the size ranges from 400-450nm, SEM measures the particle size of the 200-223nm AgNPs was 98.47±2.04nm and the zeta potential measures the particle size at -32.3±0.8mV, and able to analysis the particle size less than 100nm.[19]


Aloe vera is Commonly known as a medicinal agent including anti-inflammatory activity, UV protection,

anti-arthritis, antibacterial effect and promotes wound and burn to heal, properties constituents include lignin, hemicellulose, and pectin which were used as reducing agent.[20] 50gm of Aloe vera washed with deionized water, chopped and boiled in 50ml of deionized water for 20mins, the extract was filtered. 0.3mol of AgNO3 mixed in 20ml of deionized water with 20ml of extract and stirred vigorously at room temperature for 30mins. Nanoparticles produced at 6h, size ranges from 70.70±23;79.47±22 and 161.66±530nm prepared at 100c, 150C, and 200C respectively. At 12h the sizes 95.25±23;149.55±47 and 92.02±53nm at the same temperature maintained in 6h. The leaves were effective against gram-positive (Streptococcus epidermidis) and gram-positive (Pseudomonas aeruginosa).Hydroquinones act as reduction agent [21].

Green formulation of AgNPs from Ocimum sanctum: Tulsi

Tulsi was used in the medical industry found to be a great use.[22] formulation of nanoparticles involves the collection of leaves, washed in distilled water, cut into fine pieces, these pieces were added in 100ml of distilled water and stirred at 60C for 1h. Extracts were then filtered with Whatman filter paper-1, 5ml of extract solution dissolved in 45ml of 1mM of AgNO3. Nanoparticles were brought under characteristic analysis. UV-Vis spectroscopy measures between 250 and 800nm ranges, Surface plasmon Resonance of particles found to be the peak at 413nm and an absorbance band produced at 260nm, TEM involves the analysis of particles in the range from 4-40nm with a diameter of about 14.32±2.5nm, XRD estimated the size of 15nm.[23].

Green formulation of AgNPs using Rosa rugosa (Beach rose)

Rosa rugosa Commonly used as an ornamental plant, medicinal, cosmetic, aromatherapy properties. Preparation of nanoparticles procedure involves leaves collection, washed with distilled water, 50gm leaves in 500ml Erlenmeyer flask and boiled in 250ml ultra-pure water for about 20min, for silver nanoparticles formulation 60ml solution in 1mM AgNO3 and added with 2.5ml of leaf extract, the color changes from reddish yellow to deep red showed the confirmation of AgNPs formation, formulation did within 10 mins [24,25]. Extracts were used for herbal medicines and vitamin products. AgNPs analysis involves UV-Vis spectroscopy measures the size ranges from 300-1000nm and diameter ranges from 10-35nm, other methods for analysis included as FTIR, TEM. The absorbance increases with more leaf extract concentrations, AgNPs found to be more stable at the PH from 5 to 10 [26].

Green formulation of AgNPs from Shorea tumbuggaia

Shorea tumbuggaia Plant extracts extremely used for ear-aches and outer shots in the arm [27]. The stem was dried for 10 days kept in a hot air oven at 60C for about 24-48hrs then crushed into a fine powder, 1mM of AgNO3 dissolved in plant extract and makeup to 200ml then centrifuged at 18,000rpm for 25mins. Stem bark was act as the reducing agent. The color changes from dark yellowish to brown and indicates AgNPs existence [28].

The analysis involves UV-Vis spectroscopy did by UV-2450 and measures the peak at 430nm; SEM did by Hitachi S-4500 SEM machine for image acquisition it showed the spherical shape and diameter of about 40nm, EDAX used for the analysis of weight percentage of AgNPs found to be 33.52%[30].

Green formulation of AgNPs from Tea Leaf Extract

The reaction was carried out for 12h at room temperature is reported. Here tea leaf extract acted as a reduction agent. Nanoparticles were formulation by the following procedure involves of 16g of dried tea leaves were added in 100ml of ultra-pure water in 250ml of Erlenmeyer flask boiled for 5mins, 70µl of AgNO3 (10mM) were mixed with 14.25ml of tea extract in a drop wise manner and followed by vigorous stirring, the color changes from brown color indicates the existence of AgNPs. formulation AgNPs were characterized by TEM; XRD; FTIR; thermo-gravimetric analyzer, Zeta potential analyzer, synthesized nanoparticles were found to be spherical and range in size from 20-90nm, shows the activity against E.coli. AgNPs were produced at the competence of 99.1%; 99.7%; 99.9%;

99.8%; 94.6% and 95.3% with 5%, 10%, 25%, 50%, and

100%(v/v) tea extract respectively.[31] FTIR shows that the synthesized AgNPs had the involvement of amides, carboxyl, amino group, and polyphenol. And also Synthesized AgNPs were discovered as low as compared to the PVA-coated AgNPs, uncoated; commercial AgNPs which have good stability due to their functional group of the tea extract. Synthesized nanoparticles show the antibacterial activit against E.coli.[32]

Green formulation of AgNPs using Tectona grandis

Tectona grandis, the bark had been used as an anti-bacterial compound 5-hydroxy-1,4-naphthalenedione (Juglore)shows antibacterial activity against Listeria monocytogenes and methicillin-resistant to Staphylococcus aureus (MRSA)

  1. .and used for the treatment of anemia [34]. Reducing agents in the plant constituents like carbohydrates, fats, enzyme, flavonoids, terpenoids poly benzols, and alkaloids. Seeds had collected, before washing the outer coating was stripped and cleaned, dried at ambient temperature for about 3-4 days then crushed into fine powders. 5g of powdered seeds were taken in an Erlenmeyer flask of 250ml and it was added with 50ml of double-distilled water, boiling at 80C for 15-20mins, extract was filtered using muslin cloth, 1mM of AgNO3 in double distilled water were prepared at the ratio of 1:4 and mixed below to boiling point and the reaction conducted in dark place with a help of magnetic stirrer at 800rpm, the extract turned colorless to yellow to reddish-brown color within 1h analyzed by UV-Vis spectrophotometer and scanned ranges from 300-600nm measures the maximum absorbance at 440nm[35] it showed the formation of nanoparticles, in SEM the images were blurred and could not able to take image at 100nm of particles, therefore, FESEM were did. The FESEM-EDX indicates 94 per cent of Ag and 6 per cent of oxides. TEM analysis at the ranges of 10-30nm [36].

    Green formulation of AgNPs from tomato leaf

    Leaves of rosemary (Rosmarinus officinalls) were collected and washed with sterile distilled water, 5grms of leaves were added to 100ml of distilled water and boiled for 5 mins. The extracts are filtered with Whatman filter paper and centrifuged for about 5min at 4000rpm, the supernatant used for formulation of AgNPs, 1mM of AgNO3 added in a drop wise manner in 50ml of extract and incubated for 18h at room temperature, centrifuged 10mins at 10,000rpm. The color changes from yellowish-brown to brown after 24h of incubation indicates the formation of nanoparticles. The synthesized nanoparticles were analyzed under UV-Vis spectroscopy which measures the particle size at 300-400nm in a resolution of 1nm absorbance band located around 427nm. Leaf extract acted as the reducing agent, synthesized nanoparticles harmed wheat seedling and vegetable growth of tomato and wheat plants and enhance the activity of peroxidase and MDA.[37]

    Green formulation of AgNPs using Capsicum frutescence

    Capsicum peppers are produced capsaicin is ubiquitous. The capsaicinoid alkaloids were responsible for pungency. The leaf extract reduces as well as stabilizes the Ag+ ions and which contains secondary metabolites. The crystalline form of silver nanoparticle was analyzed by XRD. The bactericidal susceptibility and antibacterial activity are achieved by the method of disc diffusion for the synthesized nanoparticles. The resulting plates observed for E.coli which indicates gram-negative strain and observed B.subtilis which indicates gram-positive strain. After they incubated the plates at 370C for 24h. The diameter of the area around the well was analysed for antibacterial activity. SEM observed the size of the AgNPs, with a range of 15-20nm. The antibacterial activity mechanism was electrostatic interactions and cell wall disruption. The synthesized AgNPs high activity against gram-negative and moderate against gram-positive.[38].

    Green formulation of AgNPs using Clitoria ternatea and Solanum nigrum

    The silver nanoparticles activity contains many materials used in medicine and in burn treatment and arthroplasty to reduce infections. It also prevents bacteria colonization on prostheses, catheters, vascular grafts, dental materials, stainless steel materials, and human skin. The Solanum nigrum extract as a reduction agent which is responsible for infant mortality. The extract was added with 1Mm AgNO3 and it was centrifuged. Then the pellet was taken and washed thrice with 5ml distilled water and it analyzed by FTIR. The enzymes and proteins are not capping with AgNPs. The range of absorbance was measured at 420-440nm. Clitoria ternatea and Solanum nigrum silver nanoparticles stood against the pathogens growth. [39]

    Green formulation of AgNPs using Leaf Extracts of Pimenta dioica (Allspice)

    The Pimenta dioica (Allspice) leaf extract was resistant to spoilage. The life span of the extract was until 3 months.

    The leaf extract of pimento dioica has antiseptic, anesthetic, and other medical properties. Several researchers have reported no beneficial results from sun-dried leaves. The extract act as a reduction agent that is mixed with AgNO3. The synthesized nanoparticles were analyzed by UV spectrophotometer and AFM. The nano-surf Easy scan-2 software was used to analyze the AgNPs from the leaf extract. The largest size of AgNPs from fresh leaves in the ratio of 1:1 and the smallest size of silver nanoparticles from dried leaves.[40]

    Green formulation of AgNPs by an oak leaf and fruit extracts (Quercus)

    The author had reported that The oak leaf extract and its fruit extract had been collected and washed with distilled water to remove the unwanted particles and sun-dried for 2 days. The dried matter was crushed with mortar and 3g of ground fruit was boiled with 100ml distilled water for 10 min in 60C. The blend of leaves and fruits was filtered using Whatman no 1 filter paper and proceed in dark at 4C. the 1ml filtrate was added with 30ml of 1Mm AgNO3. After 80 mins the resulting color appeared in deep brown. Which indicates the formulation of nanoparticles than leaf extract. The pH was adjusted using NH3PO4 and NaOH. The synthesized solution was observed by UV spectrophotometer. It showed an absorbance peak at 415 445nm. Additionally, the solution was centrifuged for 5 min at 15,000 rpm. Light yellow to brown for leaf extract and pale brown to dark brown for fruit extract depending on concentration. It showed that silver nitrate reduction to silver nanoparticles. [40].


    Based on the above discourse it can be stated that the formulation of silver nanoparticles paved the way for biomedical nanotechnology for developing effectual anti-microbial drugs in future perspective. By the emergence of green formulation, it largely scaled-up silver nanoparticle formulation over physical and chemical methods in a cost-effective and eco-friendly manner. The morphology characteristics and activity of AgNPs varies according to their reaction parameters. This review provides extensive knowledge of the green-mediated formulation of silver nanoparticles being introduced over the last ten years.


    1. Sarsar V, Selwal KK, Selwal K. 2013. Green formulation of silver nanoparticles using leaf extract of Mangifera indica and evaluation of their antimicrobial activity. J Microbiol Biotech Res. 3:2732.

    2. Banerjee P, Satapathy M, Mukhopahayay A, Das P (2014) Leaf extract mediated green formulation of silver nanoparticles from widely available Indian plants: formulation, characterization, antimicrobial property, and toxicity analysis. Bioresour Bioprocess 1(3):110

    3. Pragyan Roy, Bhagyalaxmi Das, Abhipsa Mohanty, Sujata Mohapatra 2017, Green formulation of silver nanoparticles using Azadirachta indica leaf extract and its antimicrobial study, Application Nanoscience, IS-13204-017-0621-8

    4. S.Justin Packia Jacob,(2011) formulation of silver nanoparticles using Piper longum leaf extracts and its cytotoxic activity against Hep-2 cell line, volume 91 Colsurfb 2011.11.00

    5. Mohammad Ali Nasseri, Mansoore Shahabi, Ali Allahresani, Milad Kazemnejadi, Eco-friendly bioformulation of silver nanoparticles

      using an aqueous solution of Spartium junceum flower extract, Asian journal of green chemistry, volume-3, issue-3, pages 382-390.

    6. Yldrm N., Pulatkan M., Turna . Int. J. Second Metab., 2017, 4:376

    7. Ghaffari-Moghaddam M., Hadi-Dabanlou R. Ind. Eng. Chem., 2014 20:739

    8. Henry F. Aritonang, Harry Koleangan, and Audy D. Wuntu, formulation of Silver Nanoparticles Using Aqueous Extract of Medicinal Plants' (Impatiens balsamina and Lantana camara) Fresh Leaves and Analysis of Antimicrobial Activity volume 2019, ID 8642303

    9. Marslin, K. Siram, Q. Maqbool et al., Secondary metabolites in the green formulation of metallic nanoparticles, Materials, vol. 11, no. 6, p. 940, 2018.

    10. Franci, A. Falanga, S. Galdiero et al., Silver nanoparticles as potential antibacterial agents, Molecules,

[11] vol. 20, no. 5, pp. 88568874, 2015.

  1. W. Davis and T. R. Stout, "Disc plate method of the microbiological antibiotic essay," Journal of Microbiology, vol. 22, no. 4, pp. 666670, 1971.

  2. Probin Phanjom, Madhusmita Borthakur, Reshmi Das, Shweta Dey, and Tamanna Bhuyan, Green formulation of Silver Nanoparticles using Leaf Extract of Amaranthus Viridis ISSN 0973-631x volume 6, Number 1, PP:53-59

  3. Raut, W.R., Kolekar, S.N., Jaya, R., Lakkakula, R.J., Mendhulkar, D.V., and Sahebrao, B.K.,2010, "Extracellular formulation of silver nanoparticles using dried leaves of pongamia (L) Pierre," Nano-Micro Lett, 2(2),pp.106-113.

  4. Sathyavathi, R., Krishna, B.M., Rao, V.S., Saritha, R., and Rao, N.,2010, "Bioformulation of Silver nanoparticles using Coriandrum sativum leaf extract and their Application in Nonlinear Optics," Adv. Sci Letters, 3,1 (2010).

  5. Akl M Awwad, Nidá M Salem, Amany O Abdeen1, 2013 Green formulation of silver nanoparticles using carob leaf extract and its antibacterial activity 2013,4:29

  6. Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, Fatma A (2010) Rapid formulation of silver nanoparticles using the dried medicinal plant of basil. Colloids Surf B Biointerfaces 81(1):8186

  7. Vidhu VK, Aromal A, Philip D (2011) Green formulation of silver nanoparticles using Macrotyloma uniflorum. Spectrochim Acta A Mol Biomol Spectros 83:392397

  8. Pugazhendhi A, Edison TNJI, Karuppusamy I, Kathirvel B. Inorganic nanoparticles: A potential cancer therapy for human welfare. International Journal of Pharmaceutics. 2018;539(1):104 11. 10.1016/j.ijpharm.2018.01.034. – DOI – PubMed

  9. Ahmed S, Ahmad M, Swami BL, Ikram S. A review on plants extract mediated formulation of silver nanoparticles for antimicrobial applications: A green expertise. Journal of advanced research. 2016;7(1):1728. Epub 2016/02/05. 10.1016/j.jare.2015.02.007 – DOI – PMC – PubMed

  10. Chandran et al. (2006) Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M. formulation of gold nano triangles and silver nanoparticles using Aloe vera plant extract. Biotechnology Progress. 2006;22:577583. DOI: 10.1021/bp0501423. [PubMed]

  11. Patcharaporn tippayawat, nuthakritta phormoviya (2016) Green formulation of silver nanoparticles in aloe vera plant extract prepared by hydrothermal method and their synergistic anti-bacterial activity, Peer J, vol 4. DOI 10.7717

  12. S.Monda, B.A.Mirdha, and S.C.Mahapatra, The science behind sacredness if Tulsi (Ocimum sanctum Linn), Indian journal of physiology and pharmacology, vol 53, n0.4. pp 291-306, 2009.

  13. Anita yadav, Aashima kaushik, Anuja joshi, Green formulation of silver nanoparticles using Ocimum Sanctum l. and Ocimum Americanum l. for their antibacterial potential (2018) ISSN 2250-0480,volume-8,issue-1

  14. U.K. Parashar, P.S. Saxenaa, A. Srivastava, Bioinspired formulation of silver nanoparticles, Dig. J. Nanomater. Biol. 4 (2009) 159166.

  15. S.Basu, S.K. Ghosh, S. Kundu, S. Panigrahi, S. Praharaj, S. Pande,

    S. Jana, T. Pal, Biomolecule induced nanoparticle aggregation: effect of particle size on interparticle coupling, J. Colloid Interface Sci. 313 (2007) 724734.

  16. Shashi Prabha Dubeya, Manu Lahtinenb, Mika Sillanpääa,2010, Green formulation and characterizations of silver and gold

    nanoparticles using leaf extract of Rosa rugosa, Colloids and Surfaces A: Physicochem. Eng. Aspects 364 (2010) 3441

  17. Patil, K.S. et al., (2004). Anti-ulcer activity of Shoreathumbuggaia stem bark. Journal of NaturalRemedies.

  18. P.venkateswaralu, S.Ankannagreen, GREEN formulation OF SILVER NANOPARTICLES USING SHOREA TUMBUGGAIA STEM BARK,2010, International journal of drug and development.

  19. Chandran SP, Chaudhary M, Pasricha R, Ahmad R, Sastry M. formulation of gold nano triangles and silver nanoparticles using Aloe vera plant extract, Biotechnol.Prog, 22, 2006, 577.

  20. Y. Liu, D.A. Sonshine, S. Shervani, R.H. Hurt, Controlled release of biologically active silver from nanosilver surfaces, ACS Nano 4 (2010) 69036913.

  21. Qian Suna, XiangCai, JiangweiLi, MinZheng, ZuliangChenb, Chang-Ping Yua, Green formulation of silver nanoparticles using tea leaf extract

  22. and evaluation of their stability and antibacterial activity,2014, Colloids and surfaces a: physio chem eng.aspects 444 (2014) 226-231

  23. Neamatallah A, et al. An extract from teak (Tectona grandis) bark inhibited Listeria monocytogenes and methicillin-resistant Staphylococcus aureus. Lett Appl Microbiol. 2005;41(1):946.

  24. Diallo A, et al. Effect of Tectona grandis on phenylhydrazine-induced anemia in rats. Fitoterapia. 2008;79(5):3326.

  25. Bahuguna G, et al. Green formulation and characterization of silver nanoparticles using aqueous petal extract of the medicinal plant Combretum indicum. Mat Res Express. 2016;3(7):075003.

  26. Akhil Rautela, Jyoti Rani and Mira Debnath 2019, Green formulation of silver nanoparticles from Tectona grandis seeds extract characterization and mechanism of antimicrobial action on different microorganisms, Rautela et al. Journal of Analytical Science and Technology (2019) 10:5.

  27. Fatma Aly Farghaly1 and Nivien Allam Nafady, Green formulation of Silver Nanoparticles Using Leaf Extract of Rosmarinus officinalis and Its Effect on Tomato and Wheat Plants 2015, Journal of Agricultural Science; Vol. 7, No. 11; 2015 ISSN 1916-9752 E-ISSN 1916-9760

  28. ThangarajShankara, PerumalKarthigaa, KalaiyarSwarnalathaa, KalaiyarRajkarb, Green formulation of silver nanoparticles using Capsicum frutescence and its intensified activity against E. coli,2017 Resource efficient technologies, volume 3, Issue 3, pp:303-308

  29. Manish dubey, Seema bhadauriaa, B.S. Kushwah, Green formulation of nanosilver particles from extract of eucalyptus hybrida (safeda) leaf,2009, Digest Journal of Nanomaterials and Biostructures NO:3, VOL 4 PP:537-543

  30. Akshay Rajeev Geetha, Elizabeth George, Akshay Srinivasan, and Jameel Shaik1,2013, Optimization of Green formulation of Silver Nanoparticles from Leaf Extracts of Pimenta dioica (Allspice), The Scientific World Journal Volume 2013, Article ID 362890, 5 pages.

  31. Narayanaswamy Krithiga, Athimoolam Rajalakshmi, and Ayyavoo Jayachitra,2015, Green formulation of Silver Nanoparticles Using Leaf Extracts of Clitoria ternatea and Solanum nigrum and Study of Its Antibacterial Effect against Common Nosocomial Pathogens, Journal of Nanoscience Volume 2015, Article ID 928204, 8 pages.

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