IJERT-EMS
IJERT-EMS

Anti-Microbial, Anti-Oxidant Activity and Phytochemical Screening of Polyphenolic Flavonoids Isolated from Peels of Ananas Comosus


Anti-Microbial, Anti-Oxidant Activity and Phytochemical Screening of Polyphenolic Flavonoids Isolated from Peels of Ananas Comosus
Authors : Namrata, Yash Sharma, Tripti Sharma
Publication Date: 27-09-2017

Authors

Author(s):  Namrata, Yash Sharma, Tripti Sharma

Published in:   International Journal of Engineering Research & Technology

License:  This work is licensed under a Creative Commons Attribution 4.0 International License.

Website: www.ijert.org

Volume/Issue:   Volume. 6 - Issue. 09 , September - 2017

e-ISSN:   2278-0181

 DOI:  http://dx.doi.org/10.17577/IJERTV6IS090176

Abstract

Ananas comosus, commonly called pineapple is a very promising medicinal plant, which is being conventionally consumed by the people because of its delicious taste as well as high nutritional content. The endeavour of the present work conducted was to phytochemically screen the extracts made from the peels of the fruit as well as to evaluate the antimicrobial and antioxidant activity of the extracts made in different solvents. Methods: Both antioxidant and anti microbial activity of the plant extract was analyzed with the final concentration of 30mg/ml. Escherichia coli, Bacillus amyloliquifaciens, Pseudomonas aureginosa and Staphylococcus aureus were used to determine the antimicrobial activity of the extracts prepared. Electron Transfer Assay, Enzymatic and non enzymatic assays were evaluated by various antioxidant methods. Results: Study reveals that flavonoids were screened positive in extracts made in ethanol, methanol, n-hexane and acetone. Highest antimicrobial sensitivity was observed in Ethanolic extract with an inhibition zone diameter of 15mm, 14mm, 14mm and 21mm against Bacillus amyloliquifaciens, Staphylococcus aureus, Pseudomonas aureginosa and Escherichia coli respectively. As far as antimicrobial activity by broth dilution method is concerned, Escherichia coli was the one which showed maximum percentages of inhibition i.e.98%, highest amount or proteins leaked was found to be in Staphylococcus aureus i.e. 400μg/ml of extract and maximum absorbance for nucleic acid leakage was observed in Escherichia coli, that was 0.085 at 595nm. Electron Transfer assay was performed by Total Flavonoids count and Total Phenolic count in which utmost results were given by methanolic and ethanolic extracts being 860μg/ml and6930 μg/ml respectively. All the three enzymatic activities CAT, SOD and GST were found to highest in extract made in acetone being 399.46 μmole of H2O2 consumed/min/mg of proteins, 246.15 Units/min/mg of proteins and 145.69 μmoles of CDNB-GSH conjugate formed/min/mg of proteins respectively. MDA content was found to be maximum in acetonic extract i.e. 27μg/mg of proteins. Conclusion: Peel extract of Ananas comosus have a broad antioxidant and antimicrobial activity both and have a potential in treatment of cancer and bacterial diseases.

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