IJERT-EMS
IJERT-EMS

Methods for the Preservation of Periodontal Ligament Cells using Different Storage Media


Methods for the Preservation of Periodontal Ligament Cells using Different Storage Media
Authors : Sabina Halilovic, Ahmed Osmanovic, Lejla Gurbeta, Almir Badnjevic
Publication Date: 01-09-2017

Authors

Author(s):  Sabina Halilovic, Ahmed Osmanovic, Lejla Gurbeta, Almir Badnjevic

Published in:   International Journal of Engineering Research & Technology

License:  This work is licensed under a Creative Commons Attribution 4.0 International License.

Website: www.ijert.org

Volume/Issue:   Volume. 6 - Issue. 08 , August - 2017

e-ISSN:   2278-0181

Abstract

This paper presents a review of different storage media and their efficiency in retaining periodontal ligament (PDL) cell vitality before replantation. After avulsion of a tooth, immediate replantation is recommended, however this is often not possible. Therefore, storage media that are affordable and readily available are required to avoid resorption and tooth death by preservation of the PDL cells. Numerous studies have been undergone and use of media such as Hank’s Balanced Salt Solution (HBSS), milk, aloevera, coconut water, and et cetera have been trialed. In order to find the storage media that best meets the price/quality ratio, this study compares the length of time that the aforementioned solutions for retaining PDL vitality, their pH and osmolality, and discusses their efficiency in terms of other in vitro experiments. The media that are compared in this study are Hank’s Balanced Salt Solution (HBSS), milk, aloevera, coconut water, egg albumin, and contact lens solution. Results provide that HBSS and coconut water have the longest retention time, however milk, aloevera and egg albumin are also effective for shorter periods of time.

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