Author(s): Pradipta Tokdar, Prafull Ranadive, Sunil Kumar Deshmukh, Samanta Sekhar Khora
Published in: International Journal of Engineering Research & Technology
License: This work is licensed under a Creative Commons Attribution 4.0 International License.
Volume/Issue: Volume. 6 - Issue. 07 , July - 2017
The process for the production of Coenzyme Q10 (CoQ10) from a novel fusant strain PF-P1 has been scaled up to 2 L laboratory fermenter level. At laboratory fermenter level, the strain PF-P1 produced 113.68 mg/L of CoQ10 at 96 h using fed batch [intermittent dosing of para Hydroxy Benzoic Acid (pHBA) at a concentration of 25 mg/L (final concentration) at 24 h followed by 30% of sucrose solution at 48 h and 72 h respectively] fermentation with optimized production medium containing carbon source as cane molasses and showed specific CoQ10 content of 2.4448 mg/g of dry cell weight (DCW). Optimized process resulted in significant improvement in biomass, CoQ10 titer and specific CoQ10 content than shake flask fermentation with PF-P1 strain.
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